Evaluation of antiulcer and thrombolytic activity of. This makes it harder for food to go from the babys stomach into the. Hypertrophic pyloric stenosis pediatrics merck manuals. Rats prefed for two weeks on diets from low incidence areas developed significantly fewer rumenal ulcers after pyloric ligation than rats fed on diets from high incidence areas. The animals were ether anaesthetized and the pyloric portion of the stomach was ligated after lifting it through a midline incision starting just below the. Ligating the stomach on the antrum did not change the gastric secretory pattern from that obtained by pylorus ligation. Hypertrophic pyloric stenosis pyloromyotomy care guideline.
Antiulcer activity of pisonia aculeata on pylorus ligation. In pyloric ligation induced ulcer model, the studied parameters were gastric volume, ph, total acidity, free acidity, and ulcer index whereas in ethanol and cysteamine induced ulcer model, the ulcer index was determined for severity of ulcers. About two cases volume 2 issue 2 2015 y ben ahmed, s ghorbel, a charieg, f nouira, r khemekhem, s jlidi and b chaouchi department of pediatric surgery, tunis medical school, tunisia corresponding author. The ulcer index is determined 19 hours after pylorus ligation. Pylorus ligation alters the function of cytoprotective prostaglandin through the accumulation of gastric juice and, thereby, induces gastric ulcer singh et al. Ocimum sanctum os is known to possess various therapeutic properties. Ligation was done without causing any damage to stomach. Local administration of atropine, mecamylamine, and cocaine into the lumen of a pylorus sac inhibited gastric secretion. Pdf zusammenfugen pdfdateien online kostenlos zu kombinieren. The prevalence of gastric cancer associated with lynch syndrome ls is highly variable, and the underlying histologic pathway or molecular mechanisms remain unclear. Lipid peroxidation product mda, antioxidant contents and secretory activity have been studied in the rat stomach at different time intervals after pylorus ligation. The protective action was found in various individual items of food taken from the diets of low incidence areas. Gross and histomorphological study of antiulcerogenic. The pylorus is a muscle that opens and closes to allow food to pass through the stomach into the intestine.
To study the antisecretory and antiulcer activity of asparagus racemosus willd. Pyloric stenosis is a rare condition that makes the valve between a newborns stomach and small intestine get thick and narrow. Gastroprotection against pylorus ligation induced ulcer pl pylorus ligation pl on the fasted rat stomach was done under anaesthetized conditions using pentobarbitone 35mgkg, i. Male babies, especially firstborn males, are more at risk than females. They are deprived of both food and water during the postoperative period, rats were sacrificed by an over dose of anaesthetic ether after four hours of pyloric ligation. I am proposing a merge from the following articles into this article. Chromatin interaction analysis with updated chiapet tool v3. Zaghlool ss, shehata ba, aboseif aa, ellatif haa 2015 comparison between the protective effects of famotidine, ginger and marshmallow on pyloric ligation induced peptic ulcer in rats. After four hours, the rats stomachs were removed and gastric juice and blood samples were. Mechanism of pylorus ligation and ethanol induced ulcer. Get a printable copy pdf file of the complete article 1. The abdomen was opened, cardiac end of the stomach was dissected out and the contents were drained into a. The mechanism of gastric hyperacidity produced by pylorus.
Pediatric hypertrophic pyloric stenosis surgery treatment. Antioxidants free fulltext gastroprotective and anti. Jul 26, 2017 surgical repair of hypertrophic pyloric stenosis hps is fairly straightforward and without many complications, yet properly preparing the infant for this procedure is vitally important. Available online through ijpbs volume 1 issue 4 octdec. The pyloric canal lengthens, the whole pylorus thickens, and the mucosa becomes oedematous causing functional obstruction of the gastric outlet. Pylorus ligation caused ultrastructural changes in the gastrin cells of the pyloric gland area and in the histaminestoring ecl and alike cells of the oxyntic gland area. Nov 28, 1999 hypertrophic pyloric stenosis in infants following pertussis prophylaxis with erythromycin knoxville, tennessee, 1999 stenosis continuedin february 1999, pertussis was diagnosed in six neonates born at hospital a in knoxville, tennessee. Effect of caesalpinia bonducella extract in pylorus ligation induced ulcers in wistar rats odelia sawian 1, girish n. Pdf therapeutic potential of fk 506 in pyloric ligation. Normal electrolytes and no evidence of dehydration 5% dextrose with 0. This study aims to investigate the protective effects of.
Hypertrophic pyloric stenosis in infants following pertussis. Causes, incidence, and risk factors normally, food passes easily from the stomach into the first part of the small intestine through a valve called the. As compared to the normal rats, pylorus ligation caused gastric damage with ulcer index of 4. I plan to significantly expand the article on pylorus in the future and feel that it would be better to have the canal and antrum covered in this section under a structure subheading. Mechanisms of gastric acid secretion after pylorus and oesophagus. The present investigation was undertaken to suggest the involvement of free radicals in the pathogenesis of pyloric ligation induced ulcers in rats. The parameters studied were ulcer index, gastric juice volume, ph, free acidity and total acidity. Pyloric ligation was performed by exposing the duodenum through a midline incision and tying the pyloric end of the stomach, with care not to occlude any blood vessels. Evaluation imaging expert opinion use pyloric stenosis ultrasound to confirm diagnosis pyloric muscle thickness 3. For this study albino wistar rats150200g were used. There are various methods for the production of peptic ulcers in animals and pyloric ligation is one of the most commonly used methods, 14. Pdf surgical anatomy of the innervation of pylorus in human and. Waterimmersion stress for 7, 14, or 20 hr consistently induced linear or punctate stress ulcers mucosal erosions in the corpus of the stomach in intact rats.
Progressive hypertrophy of the pyloric sphincter resulting in obstruction. Four hours after pyloric ligation, animals were sacrificed by cervical dislocation under anesthesia. The abdomen was opened and the ligation was made at the pyloric end without causing any damage to the blood supply. Hypertrophic pyloric stenosispyloromyotomy care guideline. We evaluated its antiulcerogenic activity in cold restraint cru, aspirin asp, alcohol al, pyloric ligation pl induced gastric ulcer models in spraguedawley rats, histamineinduced duodenal hst ulcer in guinea pigs, and ulcerhealing activity, in acetic acidinduced ac chronic ulcer model. Stomachs were isolated and the gastric juice was collected and its volume was measured. Policy and ethical obligation for postpartum sterilization article pdf available in obstetrics and gynecology international 45 june 2016 with 145 reads how we measure reads. Pyloric stenosis measurements vary from publication to publication but general ballpark figures are presented here.
Original article antisecretory and antiulcer activity of acacia catechu against indomethacin plus pyloric ligation induced gastric ulcers in rats. The acid response to pylorus ligation in vagally intact rats was unaffected by. Gastroprotective and antioxidant potential of althaea o. The gastric juice was collected and centrifuged at rpm for 10 minutes. Pyloric ligation causes accumulation of the gastric juice and hindrance of the gastric blood circulation, which lead to damage of the upper gastrointestinal tract and the formation of ulcers 16, 18. Most cases of pyloric stenosis are diagnosedconfirmed with ultrasound, if available, showing the thickened pylorus and nonpassage of gastric contents into the proximal duodenum. This document may contain data, which exceeds the sheet parameters. Because a healthcare worker at hospital a was most likely the. Oct 14, 2014 screening of anti ulcer drugs dr divya krishnan 1. Y ben ahmed, pediatric surgeon, department of pediatric surgery, tunis medical school.
Infantile hypertrophic pyloric stenosis slideshare. Sharath s s1, preethy j1, surendra kumar g1, revannaswamy a1, muralidhar p1, sujith tumkur rajashekar3, prabhavathi2, rohini kr1. Pyloric definition of pyloric by medical dictionary. Two animals, which had had no pyloric ligation, each received 18 mg. Our pdf merger allows you to quickly combine multiple pdf files into one single pdf document, in just a few clicks.
Effect of dimethylglycine on gastric ulcers in rats. These capillaries merge to form the bile ducts and eventually become the hepatic duct. Doctors give unbiased, trusted information on the use of surgery for pyloric stenosis. Bansode 2 1department of pharmacology, bharati vidyapeeth deemed university, poona college of pharmacy, pune, maharashtra, india. The ethanolic extract of ficus recemosa was evaluated for its antiulcer activity against pyloric ligation, aspirin and ethanol induced gastric ulcer in rats. Box b continue en at 25 mlhr for 24 hrs before increasing. The extract was administered to the overnight fasted rats, one hour prior to aspirin or alcohol or pyloric ligation challenge.
Comparative evaluation of the efficacy of cardiospermum. The stomachs were removed and the animals which were not already dead were killed. Antiulcer activity of ethanolic extract of salvadora. Four groups of rats were selected containing six animals in each group.
Therapeutic efficacy of the root tubers of aconitum. Management of pyloric stenosis initial assessment diagnosis abcde approach assess baseline observations pews step 2 fluids correct any hypovolaemia. The initial symptom is vomiting, mild at first but becoming increasingly more forceful. Protective effect of ginger oil on aspirin and pylorus. Pyloric ligation was performed using the standard protocol after one hour of drug administration shay et al. Pyloric ligation induced ulcer albino rats female were divided into four groups of six animals each. Mechanisms of gastric acid secretion after pylorus and. The advantages and disadvantages of each approach will be discussed, with particular reference to the decisions that lead to a choice of feeding method algorithm 1. Procedure female wistar rats weighing 150170 g are starved. The gastric ulcer score was recorded according to the method described by aguwa. The ulceration is caused by accumulation of acidic gastric juice in the stomach.
Investigations on gastroprotective effect of citalopram. Evaluation of antiulcer activity of moringa oleifera seed. The second, third, fourth groups received the ethanol extract of c. In india there are regions of high incidence and regions of low incidence of duodenal ulcer. In the denervated rats the acid response to pylorus ligation was blocked by drainage of the stomach and therefore probably caused by distension, a mechanism which is independent of the vagal impulse flow. Pyloric stenosis, also called infantile hypertrophic pyloric stenosis, is a condition caused by an enlarged pylorus. Jul 09, 2019 pyloric stenosis ps is the most common pediatric surgical disorder of infancy that requires surgery for associated emesis. Blood samples were collected from the retinoorbital. Pylorus ligation causes accumulation of acid and pepsin, which leads to auto digestion of gastric mucosa and ulceration. The mechanism of the inhibition of gastric secretion. Animals were anesthetized by intraperitoneal injection of ketaminexylazine 50 mg kg at 1. To study the antiulcer activity of aqueous extract of roots of tephrosia purpurea aetp using different models of gastric and duodenal ulceration in rats. Introduction peptic ulcer disease is one of the most common gastrointestinal disorders, which causes a high rate of morbidity falk, g. There are many drugs used for the treatment of gastric ulcer, but most of these produce several adverse reactions.
Pyloric duplication with communication to the pancreas in a neonate simulating hypertrophic pyloric stenosis july 2011 journal of pediatric surgery 467. Guidelines for the management of pyloric stenosis investigations test feed capillary blood gas cbg. Pyloric stenosis infantile hypertrophic pyloric stenosis ihps is a condition that effects young infants. Pyloric ligation was done by ligating the pyloric end of stomach of rats on third day under pentobarbital anesthesia at a dose of 35 mgkg of body weight after 45 min of extract and omz treatment shay et al. The gastric lining was examined macroscopically for the presence of ulceration. Therefore, the present work evaluates the gastroprotective effect of citalopram against the cold restraint stress crs and pyloric ligation pl induced gastric ulcer models.
The response to pylorus ligation in innervated rats was. Pylorus ligation significantly increased gastric acid concentration over that found in nonligated acute fistula rats. Animals were fasted for 24 h before the study, but had free access to water. Antiulcer activity of aetp was studied in rats in which gastric ulcers were induced by oral administration of ethanol or 0.
In the distilled water 10 mgkg, orally treated control group pyloric ligation produced ulcers in 100% animals. This increase in the gastric acid secretion causes ulcers in the stomach. Hypertrophic pyloric stenosis may cause almost complete gastric outlet obstruction. Pyloric stenosis complications after surgery answers on. Pdf pharmacological study of effect of some herbal. Signifi cant controversy exists regarding the best surgical treatment for complex duodenal injuries. On the 15th day, animals, excluding the normal control group, were exposed to pyloric ligation followed by indomethacin injection. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Muscle wall thickness 3 millimeters mm or greater and pyloric channel length of 15 mm or greater are considered abnormal in infants younger than 30 days. In the current new pylorus ligation model, the maintenance of experimental animals under anesthesia showed a more sensitive response to the administration of pentagastrin and histamine secretagogues, as statistical difference in the three parameters evaluated was obtained with smaller doses of histamine and pentagastrin tested, namely 3 mgkg.
Several factors are implicated in the pathogenesis of gastric ulcer including increased acidpepsin. Infantile hypertrophic pyloric stenosis ihps is a disorder of young infants caused by hypertrophy of the pylorus, which can progress to nearcomplete obstruction of the gastric outlet, leading to forceful vomiting. Effect of caesalpinia bonducella extract in pylorus. After 45 min administration of extract and omeprazole, pyloric ligation was done by. Pyloric stenosis happens in babies when they are between two and six weeks of age, and an operation is needed to fix the problem. Evaluation of antiulcerogenic and ulcerhealing properties. Pyloric ligation induced ulcer the effects of aero7d at various doses were studied in pylorus ligated gastric ulcer model in rats were shown on table 1. A new model of stress ulcer in the rat with pylorus ligation. Russo on pyloric stenosis complications after surgery. The pylorus becomes abnormally thickened and manifests as obstruction to gastric emptying. Hypertrophic pyloric stenosis is obstruction of the pyloric lumen due to pyloric muscular hypertrophy. Gastroprotective and antisecretory effect of pepup tablet on pyloric ligation induced gastric ulcer model in rats hardik soni1, sweta patel 2, arindam paul, ghanshyam patel1 1 vasu research centre, a division of vasu healthcare pvt.
The present investigation was performed in aspirin and pylorus ligation induced ulcer model in wistar rats, in which ability of ginger oil to provide gastric protection was studied at two different doses, 0. It is being released in the interest of making available as much information as possible. A small midline incision was made on the abdomen of animals to access the pyloric part of the stomach. Pdf ginger and marshmallow extracts can protect against. The study was carried out to find out the antiulcer activity and thrombolytic activity of aqueous extract of tiliacora acuminate. Gastric and duodenal antiulcer effects of aqueous bark extract of.
Feb 28, 2014 introduction pyloric stenosis, or infantile hypertrophic pyloric stenosis ihps is a condition characterised by hypertrophy of the two muscle layers of the pylorus. General disclaimer one or more of the following statements. Pyloric duplication with communication to the pancreas in a. Contents introduction requirements of an ideal screening model ideal animal for screening animal models summary.
The original shay rat model involves fasting of rats for 72 hours followed by ligation of pyloric end of the stomach. After 4 hours of pyloric ligation all the animals were sacrificed to observe gastric lesions. Pdf pyloric gland adenoma in lynch syndrome semantic. Gastroprotective and antisecretory effect of pepup. One or more of the following statements may affect this document this document has been reproduced from the best copy furnished by the organizational source. Most infants with hypertrophic pyloric stenosis do not have complete gastric outlet obstruction and can tolerate their inherent gastric secretions. The present study has been designed to explore the therapeutic potential of fk506 in pyloric ligation induced peptic ulcer at different dose 1, 2 and 3 mgkg levels. Pylorus ligation in rats shay purpose and rationale a simple and reliable method for production of gastric ulceration in the rat based on ligature of the pylorus has been published by shay et al 1945. The use of pyloric exclusion for treating duodenal trauma. Characterization of a refinement of the pylorus ligation. However, inhibition of nos activity by l name 10 mg kg. Infants with ihps are clinically normal at birth, and subsequently develop nonbilious forceful. The chiapet tool v3 is a computational package for processing the dna sequence data generated from the chiapet experimental method, and it consists of 7 steps figure 3. Haeso hydroalcoholic extract of scindapsus officinalis.
Similarly, in the pylorusligated group, dag compared effectively with atropine 1 mgkg and ranitidine 100 mgkg. Bauhinia variegata, antiulcerogenic, pyloric ligation, aspirin plus pyloric ligation. In vitro, in vivo and in silico antiulcer activity of ferulic acid. Pdf to clarify the innervation of the antropyloric region in humans from a clinicoanatomical perspective. The study was carried on different gastric ulcer models and ulcers were induced by pyloric.
The preliminary phytochemical investigation, estimation of phenolic and flavonoid content and invitro antioxidant activity were. The two endocrine cell types in the oxyntic gland area were enlarged by pylorus ligation, more so after 16 hr than after 4 hr. From 1995 to 2012, 15 patients had been treated for both gastric and colonic adenocarcinomas and diagnosed as ls. Gastroprotective activity of polyphenolicrich extract of. Possible dietary protective factors in relation to the. Postpyloric feeding is recommended for more severely ill patients jiyong, et al. Gastric ulcer is one of the most serious diseases over wide the world. Oral administration of ocimum sanctum extract in the dose of 50mgkg did not exhibit protection against ulcers and there was no significant decrease. Animals in the control group received only 3% gum acacia solution 5mlkg. Autopsy findings of pyloric stenosis were first reported by blair in 1717, but it was not until 1887, when hirschsprung presented unequivocal clinical and autopsy findings of pyloric stenosis in 2 infants, that this ent. Antiulcer activity of ethanolic extract of salvadora indica. The rats were anaesthetized, the viscera were opened. It is more likely to happen in firstborn male children of caucasian families, particularly if a parent has had pyloric stenosis. Snp, larg and lps pretreatment increased the mucosal nitrite contents and protected the animals against pyloric ligation induced increase in acidity and ulcer index.
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